Ent2 equilibrative nucleoside transporter 2 aliases. Slc28 family transporters cnt display subtypeselective expression patterns. Come and experience your torrent treasure chest right here. Inhibition of an equilibrative nucleoside transporter by. Slc29 family members appear to be composed of 11 tm segments with cytoplasmic ntermini and extracellular ctermini. Ent1 also known as solute carrier family 29 member 1, slc29a1 mediates both influx and efflux of nucleosides across the membrane, including adenosine and many anticancer nucleoside drugs pmid. Cnt1 concentrative nucleoside transporter 1 drosophila. Slc28 and equilibrative nucleoside transporters ent.
Cnt1 is localized primarily to epithelial tissue whereas cnt2 and cnt3 have more widespread distributions. Involvement of the concentrative nucleoside transporter 3 and. In that network, nothing is more valuable than a transporter. Concentrative nucleoside transporter 1 how is concentrative nucleoside transporter 1 abbreviated. F concentrative nucleoside transporter, metazoanbacterial ipr018270. What is the abbreviation for equilibrative nucleoside transporter. Binding studies confirm that cbd binds to the equilibrative nucleoside transporter 1 with a k i dictionary. Involvement of the concentrative nucleoside transporter 3. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. Slc29, as well as possibly a yetunidentified vesicular transporter. Nucleoside transporters nts are a group of membrane transport proteins which transport nucleoside substrates like adenosine across the membranes of cells andor vesicles. Tgat differs from the mammalian equilibrative and concentrative transporters in three notable characteristics.
This gene is a member of the equilibrative nucleoside transporter family. Concentrative nucleoside transporter 1 cnt1 is a protein that in humans is encoded by the slc28a1 gene. Antislc28a2 cnt2 antibody from alomone labs is a highly specific rabbit polyclonal ab directed against the rat protein. Interpro provides functional analysis of proteins by classifying them into families and predicting domains and important sites.
The role of human equilibrative nucleoside transporter 1. Cnt1 concentrative nucleoside transporter 1 acronymfinder. The role of human equilibrative nucleoside transporter 1 on. The second is an adenosinepurine nucleoside transporter tgat with adenosine being the preferred substrate. Pdf the concentrative nucleoside transporter family slc28. Concentrative nucleoside transporter ipr008276 interpro entry. Concentrative nucleoside transporter 2 cnt2 is expressed in the apical membrane of the enterocyte and is the main transporter that contributes to absorption of purine nucleosides. Role of the human concentrative nucleoside transporter hcnt1 in the cytotoxic action of 5primedeoxy5fluorouridine, an active intermediate metabolite of capecitabine, a novel oral anticancer drug. We have 715 the transporter movie torrents for you. It also transports the antiviral nucleoside analogues zidovudine and zalcitabine.
They belong to the widelydistributed eukaryotic ent family of equilibrative and concentrative nucleoside nucleobase transporters and are distantly related to a lysosomal membrane protein, cln3, mutations in. Alomone labs is pleased to offer a highly specific antibody directed against an epitope of the rat concentrative nucleoside transporter 2. Stathams first time in the role of frank martin was the best of the three to date. There are two known types of nucleoside transporters, concentrative nucleoside transporters cnts. View and buy high purity nucleoside transporter inhibitors from tocris bioscience. Equilibrative nucleoside transporter 1 listed as ent1. Our finding that pfnt2 localizes to the er is a first for a member of the ent family and suggests a novel role for this organelle in. Nucleobase transport by human equilibrative nucleoside. The first is an adenosine transporter and the second is. Definition of equilibrative nucleoside transporter 1 in the dictionary. Slc29 mediate the uptake of natural nucleosides and a variety of nucleosidederived drugs, mostly used in anticancer therapy. Nucleoside transporter inhibitors tocris bioscience. Structural basis of nucleoside and nucleoside drug.
Additionally, human concentrative nucleoside transporter cnt 3. Slc28a1 sodiumnucleoside cotransporter 1 homo sapiens. Slc29 mediate the uptake of natural nucleosides and a variety of nucleoside derived drugs, mostly used in anticancer therapy. Slc29a1 equilibrative nucleoside transporter 1 mus.
Human equilibrative, nitrobenzylthioinosine nbmprsensitive nucleoside transporter ent1 was the first identified member of the ent family. Equilibrative nucleoside transporter 1 how is equilibrative. In general, two distinct purine nucleoside transporters have been identified in most parasites studied. Ent abbreviation stands for equilibrative nucleoside transporter. Different nterminal motifs determine plasma membrane targeting of the human concentrative nucleoside transporter 3 in polarized and nonpolarized cells. Ent2 slc29a2 is a sodiumindependent, nitrobenzylmercaptopurine riboside nbmprinsensitive transporter that participates in the transport of purine and pyrimidine nucleosides and nucleobases. Resistant to dipyridamole and dilazep inhibition anticancer chemotherapeutics drugs. Emerging roles of nucleoside transporters pharmacology frontiers. Concentrative nucleoside transporter 1 hcnt1 promotes. Expression and functional activity of nucleoside transporters. This definition appears very rarely and is found in the following acronym finder categories. Information and translations of equilibrative nucleoside transporter 1 in the most comprehensive dictionary definitions resource on the web. The antibody is not recommended for use on human samples. Abstractconcentrative nucleoside transporters cnts are responsible for cellular entry of nucleosides, which serve as.
Recently, a splice variant of human concentrative nucleoside transporter 3 was localized exclusively to the endoplasmic reticulum where it was shown to mediate the accumulation of nucleosides and nucleobases. Ent are described as broadspectrum equilibrative nucleoside transporters, while ent4 is primarily a polyspecific organic cation transporter at neutral ph. How is concentrative nucleoside transporter 1 abbreviated. Concentrative nucleoside transporter 1 infogalactic. Rosetta broker for membrane protein structure prediction. Concentrative nucleoside transporter 1 how is concentrative. Pfnt2, a permease of the equilibrative nucleoside transporter. Necessary for nucleoside metabolism by salvage pathways, nts are also critical determinants of pharmacologic.
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool. The transporter was the first englishlanguage feature for hong kongbased director corey yuen, who along with directing a number of hk action flicks designed fight choreography for several. Concentrative nucleoside transporter 1 cnt1 is a protein that in humans is encoded by the slc28a1 gene see also. Seung yong hwang, tae sung kim, concentrative nucleoside transporter 3 as a prognostic indicator for favorable outcome of t8. The transporter series is being revived this year with a brand new prequel to the popular action series, appropriately titled the transporter. Unlike other methods for docking ligands to the rigid 3d structure of a known protein receptor, glide approximates a complete systematic search of the conformational, orientational, and positional space of the docked ligand. The equilibrative nucleoside transporter family, slc29. Cnt2 is defined as concentrative nucleoside transporter type 2 very rarely. Mediates both influx and efflux of nucleosides across the membrane equilibrative transporter. What does equilibrative nucleoside transporter 1 mean. Predictable prognostic factor of cd56 expression in. Antislc28a1 cnt1 antibody from alomone labs is a highly specific rabbit polyclonal ab directed against the rat protein. Equilibrative nucleoside transporter 1 infogalactic.
Nts belong to solute carrier families 28 and 29 slc28 and slc29, which encode concentrative nucleoside transporters cnt and equilibrative nucleoside transporter proteins ents, respectively. Human equilibrative nucleoside transporters hents and human concentrative nucleoside transporters hcnts in the human choroid plexus hcp play a role in the homeostasis of adenosine and other naturally occurring nucleosides in the brain. Human ent1 hent1, slc29a1 was cloned from the placenta in 1996 and thus the first member to be characterized in the slc29 family 2. The 2nd transporter relied too heavily on formulaic stunt and special effects to keep the movie exciting. Human concentrative nucleoside transporters include slc28a1, slc28a2 and slc28a3 proteins. Binding studies confirm that cbd binds to the equilibrative nucleoside transporter 1 with a k i transporter torrent results. Predictable prognostic factor of cd56 expression in patients. It is sensitive es to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside nbmpr and is sodiumindependent. Nucleoside transporters nts are integral membrane proteins implicated in the salvage of natural nucleobases and nucleosides for nucleic acid synthesis. The endogenous substrates are typically nucleosides, although some family members can also transport nucleobases and organic cations. Transporter 0 hours and 10 minutes movie 20 a new breed of criminals have created a cult like network of human traffickers. The wellknown rosetta program and one of its protocols named broker was used in two test cases. Nucleoside transporter an overview sciencedirect topics.
It has been designed to recognize cnt2 from rat and mouse samples. The endogenous substrates are typically nucleosides, although some family members can. Concentrative nucleoside transporter 1 listed as cnt1. Since human nucleoside transporters hnts were identified by their. Involvement of the concentrative nucleoside transporter 3 and equilibrative nucleoside transporter 2 in the resistance of tlymphoblastic cell lines to thiopurines. The role of human equilibrative nucleoside transporter 1 on the cellular transport of the dna methyltransferase inhibitors 5azacytidine and cp4200 in human leukemia cells johanna hummeleisenbeiss, antje hascher, petterarnt hals, marit liland sandvold, carsten mullertidow, frank lyko and maria rius. The human slc29 family of proteins contains four members, designated equilibrative nucleoside transporters ents because of the properties of the firstcharacterised family member, hent1. Crystal structure of a concentrative nucleoside transporter from vibrio cholerae at 2. Predictable prognostic factor of cd56 expression in patients with acute myeloid leukemia with t8. They belong to the widelydistributed eukaryotic ent family of equilibrative and concentrative nucleosidenucleobase transporters and are distantly related to a lysosomal membrane protein, cln3. Slc28 and slc29 families consist in three and four members. Nucleoside transporters are divided into two families. Nucleoside transporters are divided into two families, the sodiumdependent, concentrative solute carrier family 28 slc28 and the equilibrative, solute carrier family 29 slc29.
Jun 28, 2003 the human slc29 family of proteins contains four members, designated equilibrative nucleoside transporters ents because of the properties of the firstcharacterised family member, hent1. Cnt2 stands for concentrative nucleoside transporter type 2. Pmid238575 73112 shh errastimurugarren e, casado fj, pastoranglada m. Equilibrative nucleoside transporter 1 ent1 is a protein that in humans is encoded by the slc29a1 gene. The concentrative nucleoside transporter cnt family slc28 has three members. Alternative names concentrative nucleoside transporter 1. Equilibrative nucleoside transporter, concentrative nucleoside transporter 2, concentrative nucleoside transporter 1, concentrative nucleoside transporter, equilibrative nucleoside transporter 1, equilibrative nucleoside transporter 2, equilibrative nucleoside transporter family. Ent1, ent2 and ent4 are cellsurface transporters, while ent3 is intracellular, possibly lysosomal. Gray jh, mangravite lm, owen rp et al 2003 functional and genetic diversity of concentrative nucleoside transporter 1, cnt1, in human populations. Cnt1 stands for concentrative nucleoside transporter 1. Your top supplier for concentrative nucleoside transporter research.
Slc28 and slc29 families consist in three and four. Mutual role of ecto5nucleotidasecd73 and concentrative. Cnt1 is defined as concentrative nucleoside transporter 1 somewhat frequently. The cnt1 and cnt2 transporters are coexpressed in liver.
Crystal structure of a concentrative nucleoside transporter from. The concentrative nucleoside transporter family slc28. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. In this search, an initial rough positioning and scoring phase that dramatically narrows the search space is followed by torsionally flexible energy optimization on an. Human nucleoside transporters nts belong to two families namely the slc28 family of cationlinked human concentrative nucleoside transporter hcnt proteins hcnt1, hcnt2 and hcnt3 and slc29 energyindependent human equilibrative nucleoside transporter hent proteins hent1, hent2, hent3 and hent4 1,2. Physiologic nucleosides and the majority of synthetic analogs with antineoplastic andor antiviral activities are hydrophilic molecules that require specialized plasma membrane nucleoside transporter nt 2 proteins for passage into and out of cells 1, 6. Equilibrative nucleoside transporter 1 ipr030195 nucleoside transporter ent1 was the first identified member of the ent family. Slc28 and equilibrative nucleoside transporters ents. How is concentrative nucleoside transporter type 2 abbreviated. Jan 11, 2010 human equilibrative nucleoside transporters hents 1 3 and human concentrative nucleoside transporters hcnts 1 3 in the human choroid plexus hcp play a role in the homeostasis of adenosine and other naturally occurring nucleosides in the brain. The protein is categorized as an equilibrative as opposed to concentrative transporter that is sensitive to inhibition by. Slc28 and slc29 families of nucleoside transporters.
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